- Expr14282
mkate2::NID-1strongly localized to intestinal BM and localized to gonadal BM and nerve ring, consistent with previous reports. Interestingly, we found that EMB-9::mcherry strongly localized to BM in pharynx, although mkate2::NID-1 was faintly localized at pharyngeal BM. We also confirmed strong expression of EMB-9::mcherry in coelomocytes, but not in the strain expressing mkate2::NID-1. The mkate2::NID-1 and EMB-9::mcherry are similarly localized to BM at gonad and uterus.
- Expr14283
EMB-9::mcherry strongly localized to BM in pharynx. We also confirmed strong expression of EMB-9::mcherry in coelomocytes, but not in the strain expressing mkate2::NID-1. The mkate2::NID-1 and EMB-9::mcherry are similarly localized to BM at gonad and uterus.
- Expr14507
Type IV collagen localizes to the BM and is expressed primarily in the body wall muscles, with the neuronal ring ganglion, spermatheca, uterine mucles, vulval muscles, and the distal tip cell of the somatic gonad also contributing collagen during specific larval stages.
- Expr16208
Besides MCM-7, also CDT-1::GFP expression was consistently observed in the AC nucleus prior to and during invasion. In contrast to GFP::MCM-7, CTD-1::GFP continued to be expressed in the AC after BM breaching in late L3 larvae (Pn.pxx stage).
- Expr16320
SPC-1::GFP outlined all 7 vulval rings and was present near apical (luminal) membranes and also at sites of cell-cell contact (basolateral membranes). In contrast, UNC-70::GFP was present only at basolateral membranes, while SMA-1::GFP was present only at apical membranes. We showed previously that the spectrin-related protein VAB-10a/spectraplakin also localizes apically in the vulva (Cohen et al. 2020)
- Expr16322
SPC-1::GFP outlined all 7 vulval rings and was present near apical (luminal) membranes and also at sites of cell-cell contact (basolateral membranes). In contrast, UNC-70::GFP was present only at basolateral membranes, while SMA-1::GFP was present only at apical membranes. We showed previously that the spectrin-related protein VAB-10a/spectraplakin also localizes apically in the vulva (Cohen et al. 2020)
- Expr16323
SPC-1::GFP outlined all 7 vulval rings and was present near apical (luminal) membranes and also at sites of cell-cell contact (basolateral membranes). In contrast, UNC-70::GFP was present only at basolateral membranes, while SMA-1::GFP was present only at apical membranes. We showed previously that the spectrin-related protein VAB-10a/spectraplakin also localizes apically in the vulva (Cohen et al. 2020)
- Expr16321
SPC-1::GFP outlined all 7 vulval rings and was present near apical (luminal) membranes and also at sites of cell-cell contact (basolateral membranes). In contrast, UNC-70::GFP was present only at basolateral membranes, while SMA-1::GFP was present only at apical membranes. We showed previously that the spectrin-related protein VAB-10a/spectraplakin also localizes apically in the vulva (Cohen et al. 2020)
- Expr16207
In mid-L2 larvae, GFP::MCM-7 was expressed at comparably lower levels in the newly specified AC. During the late L2 and early L3 stage, GFP::MCM-7 expression in the AC further declined but was still detectable in mid- L3 larvae just prior to invasion. In late L3 larvae, after the BM had been breached, no GFP:: MCM-7 expression was detectable in the AC.
- Expr15292
As expected, localization of mkate2::HIM-4 to the pharyngeal basement membrane was observed at the L1 stage. In contrast to the uniform localization on the basement membrane of type IV collagen or nidogen, which are major components of the basement membrane, that of mkate2::HIM-4 was polarized at the anterior part of the pharyngeal basement membrane. A unique polarized localization pattern was also observed in the pharyngeal basement membrane at stage L4. These observations indicate that polarized HIM-4 localization in the anterior part of the pharyngeal BM is maintained throughout the L1-L4 stages. The localization was also observed at the rachis during gonadal development at the L4 stage; this observation was consistent with that of previous reports.