Expr9837

Nuclear-localized GFP was seen from the comma-stage embryo through to the mature adult in a variety of cell types. NB. Some variation was found between transgenic lines generated for the same reporter gene fusion clone. GFP was observed in many cells in embryos and from L1 to L3. Neurons in head, tail and ventral nerve cord expressed GFP, DVA particularly strongly in some larvae and adults. Muscle cell nuclei throughout the body contained GFP, with higher levels in the M lineage from the L1. In some L1s, L2s and L3s, GFP expression was observed in the P lineage although this was variable presumably due to mosaicism of the transgenic array. GFP was also seen in the developing vulva and reproductive muscles. Intestinal expression of GFP was present in L3s, L4s and adults, but inconsistently.