7 Expression patterns found (0.009 seconds)

Expr16193

In the case of developmentally arrested dauer larvae, the enzyme was found in the nerve ring and along the body wall with the apparent lack of fluorescence signal in the gut. The latter location (along the body wall) reflects presumably the enzyme protein present in the gonad primordium.

Expr2339

Reporter gene expression was observed in many nerve cells in the head ganglia, the nerve cord and in the tail ganglia. Expression was also observed in the pharynx although such expression has been attributed to use of an incomplete promoter fragment as is likely to be the case in this instance. Expression in the male tail was restricted to two nerve cells. The identities of the nerve cells have not been determined.

Expr2338

Expr8971

In first-stage larvae, GEM-1::GFP is expressed on the plasma membrane of multiple cell types. These include the somatic gonad progenitors Z1 and Z4, most or all of the cell in the pharynx, and multiple neurons in the head an tail. Expression was observed in the body-wall muscles in the case of the pEJ52 mix, but not the pEJ44 mix. This difference is probably due to the absence of introns 2 and 3 in pEJ44. GEM-1::GFP expression persists within at least a subset of somatic gonad cells in later larval stages and is occasionally detectable within the distal tip cells in adult animals.

Expr101

Staining is first observed in precomma stage embryos. In all larval stages, expression is seen sporadically, as a number of nuclei in the head and occasional rows of paired nuclei down the body. This pattern seems to be highly mosaic as the number nuclei in the head bodywall that stain varies considerably. This mosaicism is also evident in expression of the bodywall nuclei in the body. These appear to be bodywall muscle. Some of the staining in the head appears to be that of the nuclei of the pharyngeal muscles (m2, m3, m4, m5, m6, m7). This component is a common feature of expression patterns from fusions with incomplete promoters, but this does not seem to be the case in this situation. Occasional staining in the nuclei of the posterior intestine is also seen.

Expr1258

AIR-2 protein was specifically localized to meiotic chromosomes. Diffuse AIR-2 staining was found in the syncytial distal gonad and the cellularized oocytes of the proximal gonad. In the oocyte most proximal to the spermatheca, a striking localization of AIR-2 to meiotic chromosomes was found. Occasionally, faint AIR-2 staining of chromosomes was noticed in the neighboring oocyte as well. AIR-2 immunostaining of fixed gonads dissected from C. elegans males revealed that AIR-2 was also present on meiotic chromosomes in spermatocytes. AIR-2 staining was also found in mature sperm present in both males and hermaphrodites. However, in this case staining did not appear to be chromatin associated. Instead, AIR-2 appeared to be present in the cytoplasm and/or on the sperm membrane.

Expr15100

nekl-2 and nekl-3. Consistent with our previous study (YOCHEM et al. 2015), these reporters were expressed strongly within the epidermis beginning in late embryonic development. NEKL-2::NeonGreen was present in cytosolic puncta that were enriched at the hyp7-seam cell boundary, similar to our observations for MLT- 2 and MLT-4. As anticipated, NEKL- 2::NeonGreen showed extensive colocalization with MLT-2::mKate2 in the apical region of hyp7, although some puncta did not overlap. In the case of NEKL-2 and MLT-3, coexpression was more variable. In apical regions, including the region encompassing the hyp7-seam cell boundary, most NEKL-2::NeonGreen and MLT- 3::mKate2 puncta failed to overlap, although colocalization of some puncta was often observed. Moreover, in some animals we observed moderate-to-strong overlap, particularly in larger apical accumulations and in more medial planes.