unc-40p::unc-40::gfp genomic translational reporter gene is localized to all dividing ray cells before stage 1 of male development and only in ray structural cells from stage 3 onward.
smp- 2::gfp is strongly expressed in the male hook in close proximity to ray 1 in wild-type adult males. Expression was observed in rays 7, 8 and 9. No expression was observed in ray 1-6 lineages.
The alpha-PKD-2 antibodies stained only male-specific sensory neurons in wild-type animals. Based on cell body positions, the PKD-2 protein is detected in the head CEMs, ray (except Ray 6), and hook HOB neurons of wild-type males.
In hermaphrodites, the expression of Ptba-6::mCherry was observed only in the IL2 inner labial sensory neurons and the HSN motor neurons. In males, this reporter was expressed in the IL2s, the cephalic companion CEM sensory neurons, the HOB hook neuron, and all ray RnB neurons, usually excluding those of ray 6.