We observed CYK-1::GFP localized in the germline similar to endogenous CYK-1 (Severson, Baillie, & Bowerman, 2002; Figure S8a). BWM appeared grossly normal in these animals, and we did not observe CYK-1::GFP at any structure in BWM, including DBs.
DAF-19A/B were expressed only in nonciliated neurons and not in ciliated sensory neurons. In summary, DAF-19A/B are expressed in 200 to 240 nonciliated neurons.
Expression in the alimentary canal: Strong and consistent expression in pm2, pm4, pm5, mc1, mc2. Weak or rare expression in pm3, pm6, pm7, pm8, K.a/K$(B!G(B cells.
The B+B enhancer (pOK17.13) activates frequent expression only in pharyngeal muscles m3, m4, m5 and m7, with occasional expression in m1. No expression has been observed in m6 or m2.
Animals that contained mig-5::gfp expressed GFP in the B, QL cell and several cells in the nerve ring. MIG-5::GFP was expressed strongly in the B cell and its descendants.