Expr13302

Previous studies using reporters driven by the ins-9 promoter suggested that ins-9 is expressed in the ASI and ASJ amphid neurons as well as in additional tissues (Chen and Baugh, 2014; Pierce et al., 2001; Ritter et al., 2013). In contrast, in transgenic L2 larvae expressing ins-9::SL2::mNG, we consistently observed green fluorescence solely in one pair of sensory neurons. In animals in which neuronal morphology was discernable, we identified the fluorescent cells as the ASI amphid neurons. We did not observe fluorescence in more than one pair of amphid neurons in any animal, nor did we detect fluorescence in other neurons or tissues. Since ins-9::SL2::mNG contains genomic elements from the ins-9 locus that are missing from other reporters in the literature (Chen and Baugh, 2014; Pierce et al., 2001; Ritter et al., 2013), these observed patterns of expression are likely to be physiologically relevant.

Expr7986

cwn-1 was first observed in early embryos, at approximately 0-2 hours after egg laying. cwn-1 expression persisted throughout embryonic development. Baugh et al. previously showed that cwn-1 was expressed in embryonic body wall muscles derived from the C and D blastomeres. Throughout larval development cwn-1 is expressed in body wall muscles (BWMs) and ventral cord neurons (VCNs), predominately in the posterior half of the animal. In the L1 and L2 stages, cwn-1::GFP is expressed predominantly posterior to P6.p, although some animals show expression in the area of the VPCs. During the L3 and L4 stages, the domain of cwn-1 expression expands to more anterior BWMs and VCNs. Expression of cwn-1 was also observed in intestinal nuclei, and in nuclei of hypodermal cells that had joined the hyp7 syncytium, such as the VPC descendants and progeny from seam cell lineages. In late L4 and young adult animals, expression of cwn-1 was observed in the vulval muscles and in fused seam cells.