Expr12451

iffb-1 mRNA was detected by in situ hybridization at all developmental stages and was particularly abundant in early embryos and in the germ line of L4 larvae and adults. The iffb-1 mRNA expression pattern has been characterized by the Nematode Expression Pattern Database consortium (NEXTDB).

Expr9121

It is continuously expressed at high levels exclusively in all cells of the gut lineage, starting at the 2E cell stage and progressing through adulthood. The expression appears strongest during embryogenesis and diminishe somewhat after hatching, consistent with endogenous expression measured in genome-wide microarray expression studies of embryonic and adult intestines.

Expr16087

We furthermore note that a pha-4 reporter allele that we generated through CRISPR/Cas9 genome engineering is continuously expressed throughout the entire pharyngeal nervous system, at all postembryonic stages

Expr13614

The earliest expression for the gene K04A8.6, chosen based on data in the C. elegans Expression Pattern Consortium database, was also detected in 3-fold embryos. Nuclear localised expression was found in bodywall muscles, hypodermis and the pharyngeal muscles in all post-embryonic stages with the addition of vulval muscles within adults. The fluorescence was not uniform in any of these nuclei; there were regions where fluorescence was absent giving the nuclei a speckled appearance. The majority of expressing nuclei were concentrated in the anterior of the worm, with other nuclei in the tail corresponding to the tail hypodermal cells. Hyp7 and bodywall muscle nuclei were also visible. Very occasionally a punctate distribution of the fusion protein was observed in single adult bodywall muscle cells with some fluorescence remaining in the nucleus. When this non-nuclear localisation of the fusion protein was seen, the fluorescence was arranged in well-separated puncta, which were brighter than the surrounding nuclei in intensity and therefore very distinct.

Expr4818

aqp-8 localized exclusively to the excretory system of the worm. Expression of aqp-8 also appears to be localized to an additional cell. The aqp-8p::GFP-PEST-expressing worms displayed an identical spatial pattern to the worms carrying the usual aqp-8::GFP construct, but due to the short half-life of the GFP-PEST construct, authors were able to determine that aqp-8 is transcribed only in the interval between the first larval stage and early adulthood. The relative levels of expression in the excretory cell and the excretory gland cell appeared to be similar to each other. Expression patterns derived from extrachromosomal arrays may be confounded by somatic loss of the transgene (leading to mosaically expressing transgenes). Therefore, the expression pattern of aqp-8 was confirmed by generating a genome-integrated aqp-8p::GFP transgenic line to prevent the sporadic loss of the transgene in somatic tissue.

Expr16091

We tagged the kin-36 locus with a gfp::H2B::SL2 cassette at its 5' end, using CRISPR/Cas9 genome engineering. We found that kin-36 indeed displays pan-pharyngeal neuron expression; expression is also observed in all other pharyngeal cell types, but no cell types outside the pharynx, except some unidentified vulval cells.

Expr13612

The gene T03G6.3, chosen based on data from the C. elegans Expression Pattern Consortium, gave GFP expression in bodywall muscle cells of embryos at the three-fold stage of elongation and continued expression within these cells through all larval stages into adulthood, with further expression in the vulval muscles. Within embryos there was not any discernable subcellular localisation of the fluorescence but from the larval stages onwards, the fusion protein localised to distinct subcellular structures within the bodywall muscles. The distribution changed at different focal planes of the muscle, puncta being visible upon the outer regions of the cell whilst deeper within the cell the distribution appeared more uniform, interrupted with irregular areas without fluorescence. The arrangement of the puncta was consistent with the T03G6.3::GFP being localised to dense bodies. The puncta sometimes appeared to be linked with slightly thinner lines acting as connections suggesting the protein was localised all along the centre of the I-bands, but enriched in the dense bodies. There did not seem to be any fusion protein in the nucleus. The distribution of the fusion protein appeared uniform within the vulval muscles. Fluorescence in all stages was very faint in both the integrated lines obtained and all three non-integrated lines. Co-localisation experiments were performed for T03G6.3 and C46G7.2 fusion proteins to confirm the localisation sites within the sarcomere. T03G6.3 localises to the centre of the I-bands and the presence in puncta suggests that it is enriched in the dense bodies.

Expr1272

High intensity of staining of the sensory cilia.

Expr1273

High intensity of staining of the sensory cilia.

Expr13432

Expression of hsp-6 was broad and most clearly visible in the intestine. Strong expression of hsp-6 or an accumulation of the reporter led to bright staining of a posterior segment of the intestine.