Expr13289

prkl-1 is expressed in VNC neurons. In contrast to Prickle localization in other CE models (Jiang et al., 2005; Narimatsu et al., 2009; Yin et al., 2008), PRKL-1 (n = 6) was not observed to exhibit recognizable membrane or polarized subcellular localization. Relatively uniform PRKL-1 cytoplasmic localization was observed in VNC neurons.

Expr3842

daf-18 message was present predominantly in the germline in larvae and adults (93%, n = 88). This is a specific signal not detected in the daf-18 deletion mutant (n = 89) or in glp-4(bn2) animals (n = 112), where little germline proliferation occurs.

Expr4229

Pegl-1gfp is expressed in 0% of the HSNs in wild-type XX animals (n = 27).

Expr8936

UNC-17 antibody staining in AIA can be observed in 94% of wild-type animals (n=32).

Expr14531

Each of the FBF-1 and FBF-2 transgenes rescued fbf-1 fbf-2 double mutants to fertility (100%; n = 20 animals for each transgene). Rescued animals were phenotypically wild-type and FLAG-tagged FBF proteins were enriched in the distal germline, which includes the germline stem cells, consistent with their endogenous expression patterns (Lamont et al. 2004; Voronina et al. 2012). Also, their expression was essentially limited to the germline.

Expr14532

Each of the FBF-1 and FBF-2 transgenes rescued fbf-1 fbf-2 double mutants to fertility (100%; n = 20 animals for each transgene). Rescued animals were phenotypically wild-type and FLAG-tagged FBF proteins were enriched in the distal germline, which includes the germline stem cells, consistent with their endogenous expression patterns (Lamont et al. 2004; Voronina et al. 2012). Also, their expression was essentially limited to the germline.

Expr16121

We did not observe expression of endogenously tagged DLG-1::mNG protein in larval AQR (n 1⁄4 30).

Expr14679

A fosmid transgene containing sax-7p::TY1::EGFP was expressed in the DTC and localized to the membrane (n = 7/7).

Expr8940

Expressed by the male linker cell from the early L4 stage until LC death in the L4-to-adult molt (n=40/41)

Expr9069

DRAG-1::GFP was present in pharyngeal, hypodermal and intestinal cells. Robust expression in the M lineage was also detected from the 1-M to 4-M stage. DRAG-1::GFP signal was reduced (40%, n=20) or undetectable (60%, n=20) at the 8-M stage, and completely undetectable beyond the 8-M stage.