GFP::EXC-9 localized predominantly at the apical membrane of the excretory canal. Expression was also seen at lower levels within the uterine seam cell, both distal tip cells, and the lumbar ganglion. Within the canals, expression was largely coincident with the intermediate filament protein marker mKate2::IFA-4, which labels the terminal web surrounding the canal lumen (Al- Hashimi et al., 2018), though some EXC-9 was also found within the cell cytoplasm.
HSP-90 and the cochaperones DAF-41, STI-1, and PPH-5 are expressed in most C. elegans cells (Gillan et al., 2009; Richie et al., 2011; Song et al., 2009), and we confirmed their expression in the TRNs.
HSP-90 and the cochaperones DAF-41, STI-1, and PPH-5 are expressed in most C. elegans cells (Gillan et al., 2009; Richie et al., 2011; Song et al., 2009), and we confirmed their expression in the TRNs.
HSP-90 and the cochaperones DAF-41, STI-1, and PPH-5 are expressed in most C. elegans cells (Gillan et al., 2009; Richie et al., 2011; Song et al., 2009), and we confirmed their expression in the TRNs.
HSP-90 and the cochaperones DAF-41, STI-1, and PPH-5 are expressed in most C. elegans cells (Gillan et al., 2009; Richie et al., 2011; Song et al., 2009), and we confirmed their expression in the TRNs.