Expr16326

Single-copy Pnlp-17::mScarlet transgenes inserted by MosTI (El Mouridi et al., 2022) are easily visible in hermaphrodites' PVQs.

Expr10834

osta-2 was expressed neuronally, with expression in a subset of ciliated neurons, although expression was not restricted to these neuron types.

Expr11187

osta-3 was expressed neuronally, with expression in a subset of ciliated neurons, although expression was not restricted to these neuron types.

Expr2294

The anti-SQV-7 Abs stained punctate foci in the cytoplasm of several tissues in WT animals, including the vulva, seam cells, distal tip cells, and oocytes. SQV-7 Abs did not stain punctate foci in the vulval, seam cells, distal tip cells, or oocytes in animals homozygous for this sqv-7(n3789) null allele(a sqv-7 null allele that deletes most of the sqv-7 ORF).

Expr10832

osta-1 gfp expression was observed exclusively in all ciliated sensory neurons in the head amphid and tail phasmid organs, with occasional expression in other neurons. Expression was observed at late embryonic stages and was maintained throughout postembryonic development.

Expr2292

GFP was observed in many of the tissues stained by the SQV-4 Abs, including the vulva, uterus, intestine, seam, and hypodermis. GFP was absent in oocytes, probably because expression from transgene arrays is silenced in the germline. GFP was observed in vulval cells containing 10 nuclei in the early L4 stage and in vulval cells containing 14 nuclei in later L4, consistent with the pattern of Ab staining.

Expr15444

Similar to UNC-11 antibody staining, UNC-11::GFP expression appeared pan-neuronal and was also observed within coelomocytes. Not described by Nonet el al 1999, we observed GFP puncta in apical epithelial tissue just below the cuticle surface in both the head and in the body, an accumulation of UNC-11::GFP at the plasma membrane in the seam cells and weak staining in the vulval precursor cells (VPCs) at the interface between the VPCs and the vulval lumen. Finally, Nonet et al. described diffuse staining within the intestine whereas we did not observe detectable levels of intestinal expression, punctate or otherwise.

Expr2291

SQV-4 Abs stained the cytoplasm of many cells, including (but not limited to) oocytes and vulval cells, as well as uterine, seam, pharyngeal, and spermathecal cells. During the early L4 stage, 10 of the 22 vulval nuclei were in cells with dramatically increased expression of SQV-4. These 10 nuclei are the six inner nuclei of the P5.p and P7.p descendants and the four outer nuclei of the P6.p descendants. During the mid-late L4 stage and thereafter, cells containing the inner four nuclei of the P6.p descendants also increased SQV-4 expression, bringing the total of vulval nuclei that highly expressed SQV-4 to 14. Thus, the 22 vulval nuclei define three classes based on the levels and timing of SQV-4 expression.