Antisera to CAND-1 was produced in rabbits by immunization with a fusion protein expressed in E. coli comprising the C-terminal 374 amino acids of CAND-1 linked to a His tag in the pET15b vector. cand-1
The polyclonal antibodies aDEB-1/a*e and aDEB-1/ b*f were produced by Clonestar Peptide Service (Brno, Czech Republic) using synthetic peptides of the fol- lowing sequences: DEB-1/a*c: CRKLADRLNPQDR, and DEB-1/b*f: VSDHYDSSDEYD. deb-1
The polyclonal antibodies aDEB-1/a*e and aDEB-1/ b*f were produced by Clonestar Peptide Service (Brno, Czech Republic) using synthetic peptides of the fol- lowing sequences: DEB-1/a*c: CRKLADRLNPQDR, and DEB-1/b*f: VSDHYDSSDEYD. deb-1
A C-terminal fragment of SPE-15a protein (amino acids 838-1219) fused with 6 histidine residues was expressed in and purified from E. coli BL21 (DE3) using Ni-NTA resin (Qiagen 30210), then used to raise mouse monoclonal antibody. spe-15
A mouse polyclonal antiserum to a His-tagged HAM-1 fusion protein expressed in E. coli was generated and used to stain whole mounts of wild-type embryos and larvae. ham-1