Rabbit polyclonal anti-SMA-1 ABD. Antibodies against the actin-binding domain. To produce GST-tagged ABD, cDNA encoding amino acid residues 195-519 of SMA-1 (residue numbers as in isoform a, from transcript R31.1a, Wormbase version WS279) was cloned into the EcoRI and NotI restriction sites of pGEX-6P1 using primers 50-CCCGAATTCATTTCCGGAGACAAACTCGG-30 and 50-CCCGCGGCCGCTTCCTGTCTTTGAAGCTCGGC.
sma-1