WBAntibody00000978

Chicken antibody against PAR-3, from Diane Morton and Kathy Baker. par-3

WBAntibody00001574

Antibody against GLD-1, from M. Lee and T. Schedl. gld-1

WBAntibody00002264

anti-ERI-1 rabbit polyclonal antibody (a gift from T. Duchaine) eri-1

WBAntibody00001906

Polyclonal rabbit antibody against UNC-7 recombinant protein, from Drs. T. Starich and J. Shaw. unc-7

WBAntibody00003010

Rabbit polyclonal antibodies against Hsp12.1, Hsp12.2, Hsp12.3, or Hsp12.6 produced in our own lab at 1:10000 dilution in TBS-T buffer containing 5% non-fat dry skim milk. hsp-12.1

WBAntibody00003012

Rabbit polyclonal antibodies against Hsp12.1, Hsp12.2, Hsp12.3, or Hsp12.6 produced in our own lab at 1:10000 dilution in TBS-T buffer containing 5% non-fat dry skim milk. hsp-12.3

WBAntibody00003013

Rabbit polyclonal antibodies against Hsp12.1, Hsp12.2, Hsp12.3, or Hsp12.6 produced in our own lab at 1:10000 dilution in TBS-T buffer containing 5% non-fat dry skim milk. hsp-12.6

WBAntibody00003011

Rabbit polyclonal antibodies against Hsp12.1, Hsp12.2, Hsp12.3, or Hsp12.6 produced in our own lab at 1:10000 dilution in TBS-T buffer containing 5% non-fat dry skim milk. hsp-12.2

WBAntibody00002135

Rat serum EU143 was raised against the predicted C-terminal 353 residues of Ce-titin. Antigens was expressed as GST fusion proteins in E. coli. cDNAs encoding antigenic polypeptides were amplified by RT-PCR using Pfu polymerase (Stratagene) and primers containing added XhoI and EcoRI sites. The primers for EU143 were 5'-GTACGAATTCAAATTCATCTCAGTGACACCAGG-3' and 5'-GATGCTCGAGCTACCGACGAATCGTTCTTCGTTG-3', respectively. Amilified product was cloned into pGEX-6P-1 (Amersham Pharmacia Biotech).T he GST fusion proteins were expressed and purified. Milligram quantities of fusion proteins were supplied to Spring Valley Laboratories (Sykesville, MD) for antiserum production. ttn-1

WBAntibody00002134

Guinea pig serum EU145 was raised against the N-terminal 314 residues of the predicted 2.2 MDa isoform of Ce-titin. Antigens was expressed as GST fusion proteins in E. coli. cDNAs encoding antigenic polypeptides were amplified by RT-PCR using Pfu polymerase (Stratagene) and primers containing added XhoI and EcoRI sites. The 5' and 3' primers for EU145 were 5'-GTACGAATTCATGGAGGGCAACGAGAAGAAAGG-3' and 5'-GATGCTCGAGCATTGGGTCAAAGGCAGTGGTCG-3', respectively. Amplified product was cloned into pGEX-6P-1 (Amersham Pharmacia Biotech).T he GST fusion proteins were expressed and purified. Milligram quantities of fusion proteins were supplied to Spring Valley Laboratories (Sykesville, MD) for antiserum production. ttn-1