The GST fusion protein of PRL-1 (GST-PRL-1) was expressed by an E. coli Rosetta (DE3) harboring the pGex-2T-prl-1 plasmid and then purified using a GST protein purification column. After purification, the GST- PRL-1 fusion protein was digested with thrombin to excise the GST tag and purified again by GST protein purification column. Afterwards, the purified protein was collected and injected to the rabbits. After multiple immunizations of rabbits, a small amount of blood is taken to test the an- tibody potency in the serum. An antibody potency of less than 1:500 after immunization indicates successful immu- nization with the PRL-1 exogenous antigen.
ptp-4A2