Because of the high amino acid sequence identity between CYB-1 and CYB-2, antibodies against these proteins were raised against their C-terminal end using the peptides CEKINRMGQNAKVDASEME and CRMGRNLEASEAETSEME, respectively. Note that the latter peptide sequence corresponds to CYB-2.2 but the antibody also detects CYB-2.1 due to their sequence similarity. Antibody specificity was confirmed by immunoblotting.
cyb-1