WBAntibody00001323

Antibody against EEA-1, provided by Grant Barth. eea-1

WBAntibody00001956

Antibody against EEA-1, provided by Grant Barth. eea-1

WBAntibody00001438

Polyclonal antibody against against the C-terminal region of APA-2.(gift of Dr. B. D. Grant, Rutgers University) apa-2

WBAntibody00002925

The eluted protein PID-2::6xHis has been concentrated to a final concentration of 1,8 mg/ml and sent to Eurogentec for antibody production (two rabbits; 28-day Speedy protocol). pid-2

WBAntibody00002743

The polyclonal mouse anti-PLK-1 antibody was generated in house by injecting 1 mg of purified full length PLK-1 into mice, purified from serum, and used in a dilution of 1:300. plk-1

WBAntibody00002918

Anti-rHc-DHS-28 polyclonal antibody was produced by immunising Institute of Cancer Research (ICR) mice (5 weeks old, female) thrice with 0.5 mg/kg of purified rHc-DHS-28 every 7 days and the titer was determined by ELISA. dhs-28

WBAntibody00002298

The synthetic peptide HIS-24-K14me1 (N-CSDSAVVAAAVEP-K(me)-VPKAKAAK-C) was chemically synthesized and coupled with sulfo-maleimidobenzoyl-N- hydroxysuccinimide ester (Squarix biotechnology GmbH, Marl, Germany) to bovine serum albumin. 500 mg of antigen was used for immunization of three rabbits in a series of three injections. Antigen injections and resulting antiserum collections were performed by Charles River. his-24

WBAntibody00002914

GPB-1 antibodies were produced by cloning full-length gpb-1 cDNA into pGEX-6P2 (Promega), expressing GST-GPB-1 and purifying it from inclusion bodies. The protein was gel purified and injected into a rabbit (Eurogentec). The resulting antibodies were strip-purified against GST- GPB-1, using 0.1 M glycine pH 2.5 as an elution buffer. Affinity-purified antibodies were then dialyzed against PBS and kept at -20°C in 50% glycerol at 0.2 mg/ml. gpb-1

WBAntibody00002939

To generate the mouse monoclonal anti-PARG-1(2D4) antibody, the cDNA encoding for residues 1-350 of C. elegans PARG-1 (isoform A) was generated by gene synthesis (IDT) and then cloned into pCoofy31 in frame with a C-ter 6 × His tail. The resulting plasmid was expressed in E. coli BL21 cells according to standard procedures and 1 mg of purified protein was used to immunize three mice in the 'in-house' monoclonal antibody facility at Max Perutz Laboratories. parg-1

WBAntibody00003089

The MBP-domain IV fusion protein is used to raise SPARC-specific antibodies. The protein is purified by amylose affinity chromatography and dialyzed into 20 mM Tris-HCl(pH 8), 50 mM NaCl. MBP-domain IV (0.2 mg in Freund's complete adjuvant) is injected subcutaneously into a rabbit. The rabbit is boosted 2 weeks later with the same amount of protein in Freund's incomplete adjuvant. The first immune bleed is collected 2 weeks after the boost and has significant anti-MBP-domain IV reactivity by immunoblotting. ost-1