4 Antibodies found (0.084 seconds)

Polyclonal rabbit antiserum against the 25KDa C-terminal polypeptide fragment of C. elegans DNA topoisomerase I. top-1

Polyclonal rabbit antiserum against the 50KDa C-terminal polypeptide fragment of C. elegans DNA topoisomerase I. top-1

polyclonal mice anti-Ce_MMP62 antibody. The full-length open reading frame (ORF) of 1,740 bp CEMMP62 (GenBank accession number: AB007815) was amplified by PCR from the single-strand cDNA using specific primers (forward 5'- GGG ATC CAT GCG GTT GAT TTA C-3' with an in-frame BamH I restriction site, reverse 5'-GGG AAG CTT TTA ACA GGC GAT ATA-3' with an in-frame Hind III restriction site). zmp-3

WBAntibody00002858

The entire sequence encoding UBXN-6 was amplified by PCR with a cDNA clone yk1159e09 as a template, and primers ubxn6- Cold-5 and ubxn6-Cold-3, and cloned onto pCold I, yielding pMUR7. Escherichia coli BL21(DE3) was transformed with pMUR7. His-tagged UBXN-6 was overexpressed by temperature shift-down and the addition of 1 mM isopropyl-1-thio-b-D-galactopyranoside and purified by using HisTrap HP and HiTrap Q columns (GE Healthcare). Purified His-tagged UBXN-6 was pooled and used for development of antibody. Rabbit anti-UBXN-6 antisera was devel- oped by IWAKI&CO., LTD. and purified using antigen-conjugated affinity resin. ubxn-6