WBAntibody00003094

Antibodies were raised against this fraction by immunizing BALB/c mice and were used to screen an Mf cDNA expression library to identify the principal antigenic proteins. Bma-srp-2

WBAntibody00003019

The primary antibody against ATP5B (which detects C. elegans ATP-2) was custom made and suitable for the analysis of complexes using native PAGE (gift from Peter Rehling). atp-2

WBAntibody00000163

A PCR fragment encoding MIX-1 amino acids 1 to 384 was cloned into the T7 expression vector pRSET B (Invitrogen), in-frame with DNA encoding six histidines. The recombinant protein was purified by nickel-chelate chromatography and SDS-PAGE. Rabbit antisera were affinity purified against column-bound antigen. mix-1

WBAntibody00002739

A full-length cDNA encoding the VIG-1 protein was sub-cloned into pET28a vector at EcoRI and XhoI sites (pDH207) with oligonucleotides (V37: 5'-AAAGAATTCA TGAGCACTGAATACGGTTGCCAG-3' and V38: 5' - AAACTCGAGTTACTTGGCACCGAGAGCTG-3' ) using PCR, and expressed in E. coli BL21(DE3). The fusion protein was purified with a Ni-NTA column (QIAGEN) according to the manufacturer's instructions, and subjected to SDS-PAGE. The gel pieces that contained the VIG-1 recombinant protein were excised and the protein was collected through gel electro-elution and used as the immunogen for two Sprague Dawley rats at the University of Calgary Animal Research Facility. vig-1

WBAntibody00002747

A full-length cDNA encoding the VIG-1 protein was sub-cloned into pET28a vector at EcoRI and XhoI sites (pDH207) with oligonucleotides (V37: 5'-AAAGAATTCATGAGCACTGAATACGGTTGCCAG-3' and V38: 5' - AAACTCGAGTTACTTGGCACCGAGAGCTG-3' ) using PCR, and expressed in E. coli BL21(DE3). The fusion protein was purified with a Ni-NTA column (QIAGEN) according to the manufacturer's instructions, and subjected to SDS-PAGE. The gel pieces that contained the VIG-1 recombinant protein were excised and the protein was collected through gel electro-elution and used as the immunogen for two Sprague Dawley rats at the University of Calgary Animal Research Facility. vig-1

WBAntibody00002841

Full-length RPL-5, RPL-25.2, RPS-3 and RPS-0 ribosomal proteins tagged with six Histidine residues (RPL-5-His6, RPL-25.2-His6, RPS-3-His6, RPS-0-His6) were expressed in E. coli. The recombinant proteins were purified by Ni-NTA agarose beads and the eluted RPL-5-His6, RPS-3-His6 and RPS-0-His6 proteins were used to raise polyclonal antibodies in rat. For RPL-25.2-His6, a specific protein band was excised from the SDS-PAGE and used to generate polyclonal antibody in rat. All polyclonal antibodies were generated in the Antibody Center at NIBS (National Institute of Biological Sciences, Beijing, China). rpl-23A.2