BL52/B6 mice were subcutaneously primed with 100 μg FIC-1 in PBS supplemented with complete Freund's adjuvant, boosted by intraperiotenal injection 4 weeks later with 20 μg in PBS and terminally bled 5 days later. fic-1
The IMMT-1 antibody was generated by injecting His6-IMMT-1 into mice (BALB/c, 6 weeks postnatal, female) 3 times at 14-day intervals (250 μg of protein per injection) and used at 1:5,000. immt-1