WBAntibody00003020

Chicken polyclonal anti-SYP-1 -a gift from the Martinez-Perez laboratory. syp-1

WBAntibody00001755

Rabbit polyclonal antibody against cathepsin D.

WBAntibody00001560

Rabbit antibody against MEC-3, from D. Xue and M. C. mec-3

WBAntibody00000339

Monoclonal anti-myosin-heavy-chain antibody 5.6.1. (gift from D. Miller).

WBAntibody00001654

Antibody against IFG-1, from B. D. Keiper and R. E. Rhoads. ifg-1

WBAntibody00001438

Polyclonal antibody against against the C-terminal region of APA-2.(gift of Dr. B. D. Grant, Rutgers University) apa-2

WBAntibody00000834

MSP mouse monoclonal antibody. Anti-MSP antibody was from M. Kosinski and D. Greenstein, Vanderbilt University School of Medicine, Nashville, Tennessee, United States

WBAntibody00000012

Monoclonal mouse antibody against purified nematode myosin. Monoclonal antibody to myosin D. 5-17 has strong reaction to myosin heavy chain A, weak reaction with myosin heavy chain B, no reaction with myosin heavy chain C or paramyosin. myo-1

WBAntibody00002858

The entire sequence encoding UBXN-6 was amplified by PCR with a cDNA clone yk1159e09 as a template, and primers ubxn6- Cold-5 and ubxn6-Cold-3, and cloned onto pCold I, yielding pMUR7. Escherichia coli BL21(DE3) was transformed with pMUR7. His-tagged UBXN-6 was overexpressed by temperature shift-down and the addition of 1 mM isopropyl-1-thio-b-D-galactopyranoside and purified by using HisTrap HP and HiTrap Q columns (GE Healthcare). Purified His-tagged UBXN-6 was pooled and used for development of antibody. Rabbit anti-UBXN-6 antisera was devel- oped by IWAKI&CO., LTD. and purified using antigen-conjugated affinity resin. ubxn-6