WBAntibody00001873

Antibody against LIN-14. from G. Ruvkun. lin-14

WBAntibody00001870

Antibody against UNC-17, provided by Dr. K. G. Miller. unc-17

WBAntibody00001957

Rabbit antibody against RAB-11, provided by J. G. White. rab-11.1

WBAntibody00001639

Rabbit antibody against RIC-4/SNAP-25, from G. Hadwiger & M.L.N. ric-4

WBAntibody00000697

Antibody against PIE-1, a gift from G. Seydoux (Johns Hopkins School of Medicine). pie-1

WBAntibody00002703

BL52/B6 mice were subcutaneously primed with 100 μg FIC-1 in PBS supplemented with complete Freund's adjuvant, boosted by intraperiotenal injection 4 weeks later with 20 μg in PBS and terminally bled 5 days later. fic-1

WBAntibody00002884

The rabbit anti-KLP-7MCAK antibody was custom produced, validated in this study and used at 1 μg/μl. klp-7

WBAntibody00003023

The IMMT-1 antibody was generated by injecting His6-IMMT-1 into mice (BALB/c, 6 weeks postnatal, female) 3 times at 14-day intervals (250 μg of protein per injection) and used at 1:5,000. immt-1

WBAntibody00003090

A total of 700μg of purified GST::Ce-lamin protein was provided to Cocalico Biologicals (Reamstown, PA) for antibody production in two guinea pigs, CUMC-GP 27 and CUMC-GP 28. A total of four boosts were given to each guinea pig before exsanguination. lmn-1

WBAntibody00003021

Rabbits were initially immunized with 200 μg full-length His::TEV tagged PALS-5 antigen in Freund's Complete Adjuvant. Rabbits were then subsequently boosted with four separate immunizations of 100 μg antigen in Freund's Incomplete Adjuvant over a 16-week period. Approximately 25 ml of serum was collected and PALS-5 polyclonal antibody was purified with an immuno-affinity chromatography column by cross- linking PALS-5 to cyanogen bromide (CNBr)-activated Sepharose 4B gel. Antibody was eluted from the affinity column in 100 mM glycine buffer pH 2.5, precipitated with polyethylene glycol (PEG), and concentrated in PBS pH 7.4 + 0.02% sodium azide. Antibody concentration was determined by ELISA. pals-5