For HIM-3phos antibody production, synthetic HIM-3 peptides (NH2-C+RISRD(pS) PYGL(pS)QGITK-COOH) with phosphorylation at Serine 277 and Serine 282 were synthe- sized and used to immunize a guinea pig by Eurofins Japan Inc. him-3
Anti Ric-8A Ser435 phosphorylated antibodies. A rabbit (6383) was immunized with a keyhole limpet hemocyanin (KLH)-conjugated synthetic 12-amino acid peptide containing phosphorylated residues corresponding to the Ser435 site: EGQY(pS). ric-8
Rabbit anti-LIN-56 antisera were generated using purified GST-LIN-56(aa 1-322) as the immunogen, affinity purified against MBP-LIN-56(aa 1-322). lin-56