WBAntibody00000444

Antibody to ZYG-9, a gift from A. Pozniakovsky (Max-Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany) zyg-9

WBAntibody00002939

To generate the mouse monoclonal anti-PARG-1(2D4) antibody, the cDNA encoding for residues 1-350 of C. elegans PARG-1 (isoform A) was generated by gene synthesis (IDT) and then cloned into pCoofy31 in frame with a C-ter 6 × His tail. The resulting plasmid was expressed in E. coli BL21 cells according to standard procedures and 1 mg of purified protein was used to immunize three mice in the 'in-house' monoclonal antibody facility at Max Perutz Laboratories. parg-1

WBAntibody00003041

Monoclonal anti-GRIF-1 hybridoma supernatants were raised with the help of the antibody facility of the Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG; Dresden). Mice were immunized with heterologously expressed and affinity-purified FL GRIF-1 fusion proteins, containing 6xHis::SUMO::GRIF-1::GFP, originally produced in BL21 bacteria and purified to homogeneity under denaturing conditions using nickel-affinity columns. Specific immunoreactivity was established in Western blotting and immunostaining experiments using yeast-expressed proteins and worm material. grif-1