To generate the F54C9.11/RABI-8 antibodies, the sequence encoding amino acids of F54C9.11 was amplified from an N2 cDNA library and cloned into pGEX-2T for 6xHis tagging. His fusions were purified from bacteria and injected into two rats (GL Bio- chem). RABI-8 antibodies were affinity purified using the same antigen.
rabi-1