PCN-1 was detected using a commercially supplied (Bethyl Laboratories), affinity-purified rabbit antibody raised against a synthetic peptide corresponding to the amino acid sequence N-DIDSEHLGIPDQDYAVVCE-C of C. elegans PCN-1. pcn-1
The anti-IKKE-1 polyclonal antibody was raised against a peptide corresponding to the IKKE-1 b/c C-terminal region (QYFAKPTSSPSSSAK; Sigma-Aldrich). ikke-1
Antibody against C. elegans Dcp2. recognize a protein in C. elegans extracts of the predicted size for the 770-amino-acid protein (molecular weight, 85,000). dcap-2
A rabbit polyclonal antibody against a peptide in the disordered region of C. elegans gcna-1 (ZK328.4) (YPEMFDSNQKPRQKPKE) was generated by Yenzym Antibodies, LLC. gcna-1
Monoclonal antibody M44 is a mouse IgG1, generated by immunization with C. elegans homogenates, that reacts with a cell surface antigen on the PVP neurons.