The egrh-1 ORF encoding amino acids 1-120 was amplified from yk484c12-pOK165.12 and inserted into pMal2c (NEB). Antibodies were raised in rabbits D1919 and D1920 (Open Biosystems) against the MBP::EGRH-1 fusion purified from E. coli BL21 (Ausubel, 1990). EGRH-1 antibodies were purified by sequentially depleting against E. coli extracts expressing maltose-binding protein (MBP) and enriching against purified MBP::EGRH-1 bound to nitrocellulose filters.
egrh-1