WBAntibody00002148

The monoclonal ASH-2 antibody was generated by injection of a fusion protein between GST and amino acids 285-572 of C. elegans ASH-2 and was affinity-purified by Abmart (Shanghai, China). The H3K4me3 antibody (8580), the H3K4me1 antibody (8895), the H3K9me3 antibody (8898) and the H3 antibody (1791) were obtained from Abcam. The H3K4me2 antibody (07-030) was obtained from Upstate Biotechnology. ash-2

WBAntibody00002162

Anti-W10G11.20/DNC-3 rabbit serum was made against GST- fused N-terminal truncated W10G11.20/DNC-3 (49 to 171). The W10G11.20/DNC-3 fragment was amplified from a cDNA library and cloned into the entry vector pENTR201 (Invitrogen). The entry clone was recombined with pDEST15 (Invitrogen) by LR recombination. The obtained plasmid was used for protein expression in E. coli, and GST-W10G11.20/DNC-3 was purified using GST-sepharose (GE Healthcare, Pollards Wood, UK). dnc-3