A peptide fragment of UNC-63 (LNVPGKRHSKRYPC), which is located in loop C and shows low homology with the equivalent region in other nAChR subunits (13), was used to generate polyclonal antibodies in rabbits. unc-63
Antisera to CAND-1 was produced in rabbits by immunization with a fusion protein expressed in E. coli comprising the C-terminal 374 amino acids of CAND-1 linked to a His tag in the pET15b vector. cand-1
Expression vector pRSETA was used to express CeTwist protein in E.coli BL21 (lys-S) cells as a His-tagged fusion protein. CeTwist-His protein was purified with Ni affinity column in denaturing conditions. Polyclonal antibody was produced in mice. hlh-8
Polyclonal antibody against Drosophila UbcD1. This antibody also recognize C. elegans ubc-2 proteins expressed in the transformed strains and yeast UBC4 in wild-type cells.