Anti-ALLO-1a and ALLO-1b polyclonal antibodies were generated by TK Craft Corporation, Gunma, Japan against peptides corresponding to the ALLO-1a C- terminal region (QYFAKPTSSPSSSAK; Sigma-Aldrich) or ALLO-1b C- terminal region (NDPLDSLSNDEFEIIETFD; Sigma-Aldrich) and affinity-purified with the same peptides. The specificity of these antibodies was verified using immunoblotting and immunostaining of wild-type and allo-1(tm4756) strains.
allo-1