WBAntibody00003005

Rabbit polyclonal anti-SMA-1 ABD. Antibodies against the actin-binding domain. To produce GST-tagged ABD, cDNA encoding amino acid residues 195-519 of SMA-1 (residue numbers as in isoform a, from transcript R31.1a, Wormbase version WS279) was cloned into the EcoRI and NotI restriction sites of pGEX-6P1 using primers 50-CCCGAATTCATTTCCGGAGACAAACTCGG-30 and 50-CCCGCGGCCGCTTCCTGTCTTTGAAGCTCGGC. sma-1

WBAntibody00002723

ICP-1 peptide antibodies were produced and affinity purified using previously described peptides (Burrows et al., 2006; Schumacher et al., 1998). icp-1

WBAntibody00002722

AIR-2 peptide antibodies were produced and affinity purified using previously described peptides (Burrows et al., 2006; Schumacher et al., 1998). air-2

WBAntibody00001638

Rat antibody against GLP-1, from Crittenden et al. 1994. glp-1

WBAntibody00003059

Purified in house after immunisation of rabbits using the sequence in Desai et al., 2003. bub-1

WBAntibody00003095

ATFS-1 polyclonal antibodies were generated and validated as previously described (Nargund et al., 2012). atfs-1

WBAntibody00002934

Polyclonal antibodies against BUB-1, BUB-3 (amino acids 189-329), Zwilch ZWL-1 (amino acids 1-200), and Mad2 MDF-2 (splice variant Y39A2AR.30A amino acids 2-203) were generated as described previously (Oegema et al., 2001; Desai et al., 2003). zwl-1

WBAntibody00002932

Polyclonal antibodies against BUB-1, BUB-3 (amino acids 189-329), Zwilch ZWL-1 (amino acids 1-200), and Mad2 MDF-2 (splice variant Y39A2AR.30A amino acids 2-203) were generated as described previously (Oegema et al., 2001; Desai et al., 2003). bub-1

WBAntibody00002935

Polyclonal antibodies against BUB-1, BUB-3 (amino acids 189-329), Zwilch ZWL-1 (amino acids 1-200), and Mad2 MDF-2 (splice variant Y39A2AR.30A amino acids 2-203) were generated as described previously (Oegema et al., 2001; Desai et al., 2003). mdf-2

WBAntibody00002933

Polyclonal antibodies against BUB-1, BUB-3 (amino acids 189-329), Zwilch ZWL-1 (amino acids 1-200), and Mad2 MDF-2 (splice variant Y39A2AR.30A amino acids 2-203) were generated as described previously (Oegema et al., 2001; Desai et al., 2003). bub-3