Nutrient-driven O-GlcNAcylation of key components of the transcription machinery may epigenetically modulate gene expression in metazoans. Knockouts of the O-GlcNAc cycling enzymes in C. elegans are viable and fertile, allowing a global analysis of the impact of GlcNAcylation. Whole genome transcriptional profiling of the O-GlcNAc cycling mutants confirmed dramatic deregulation of genes in these key pathways. As predicted, the O-GlcNAc cycling mutants show phenotypically altered lifespan and susceptibility to UV stress.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of N2 animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of N2 animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of N2 animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of N2 animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of N2 animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of N2 animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of pept-1(lg601) animals.
A quantitative shotgun proteomics approach using 15N-based metabolic labeling was conducted at 20, 40, and 60 h during development starting with L1-larvae of pept-1(lg601) animals.