- C. elegans gene expression in response to Salmonella enterica infection and recovery by Tetracycline treatment
(Part 1) Gene expression profiles of C. elegans in response to a 120 hour S. enterica infection. Synchronized larval stage 1 (L1) animals were exposed to S. enterica SL1344 for 36, 72, 96, or 120 hours. As an uninfected control, synchronized L1 animals were exposed to E. coli OP50 for 36 hours. (Part 2) Gene expression profiles of C. elegans in response to Tetracycline-mediated recovery from 72 hour and 96 hour S. enterica infections. Synchronized L1 animals were exposed to S. enterica SL1344 for 72 hours and then shifted to E. coli HT115 plus Tetracycline plates for 24 hours to resolve the infection. Synchronized L1 animals were exposed to S. enterica SL1344 for 96 hours and then shifted to E. coli HT115 plus Tetracycline plates for 24 hours to resolve the infection.
- Transcriptionally Active Regions for modENCODE_2499
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_2548
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_457
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_461
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_462
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_657
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_660
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.
- Transcriptionally Active Regions for modENCODE_459
Miller lab's modENCODE tiling-array experiments to find Transcriptionally Active Regions. Peak-calling was done using mSTAD. These experiments are designed to detect all C. elegans transcripts by hybridizing RNA to commerically available genome tiling arrays. To maximize the chances of detecting rare transcripts with limited expression in specific cells, RNA was extracted from selected embryonic cells isolated by FACS and from postembryonic cells by use of the mRNA tagging method.