- AIN-2::GFP IP of mRNAs associated with miRISC in stage sychronized C. elegans populations
AIN-2::GFP IP were used to purify miRISC from stage sychronized C.elegans populations (Egg, L1, L2, L3 and L4 stages). The mRNA composition of the IP results and the corresponding total RNA samples were analyzed by WUSTL Caenorhabditis elegans Whole Genome 23k Oligo Array. The miRISC associated mRNAs (miRNA targets) in each stage were identified by measuring the relative enrichment of each mRNA in the IP sample versus the corresponding total RNA sample
- AIN-1 and AIN-2::GFP IP of C.elegans miRNA targets
MicroRNAs (miRNAs) regulate gene expression for diverse functions, but only a limited number of mRNA targets have been experimentally identified. We show that GW182 family proteins AIN-1 and AIN-2 act redundantly to regulate the expression of miRNA targets, but not miRNA biogenesis. Immunoprecipitation (IP) and mass spectrometry indicate that AIN-1 and AIN-2 interact only with miRNA-specific Argonaute proteins ALG-1 and ALG-2 and with components of the core translational initiation complex. Known miRNA targets are enriched in AIN-2 complexes, correlating with the expression of corresponding miRNAs. Combining IP with pyrosequencing and microarray analysis of RNAs associated with AIN-1/AIN-2, we identified 106 previously annotated miRNAs plus 9 new candidate miRNAs, but nearly no siRNAs, and more than 3500 potential miRNA targets including nearly all known ones. Our results demonstrate an effective biochemical approach to systematically identify miRNA targets and provide valuable insights regarding the properties of miRNA effector complexes. Keywords: IP microarray of miRNA targets
- Mean FPKM value of all sratti control samples
This is the mean value of the N= RNASeq FPKM expression values for this gene in all life-stages of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Mean FPKM value of all tmuris control samples
This is the mean value of the N= RNASeq FPKM expression values for this gene in all life-stages of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Median FPKM value of all sratti control samples
This is the median value of the N= RNASeq FPKM expression values for this gene in all life-stages of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Median FPKM value of all tmuris control samples
This is the median value of the N= RNASeq FPKM expression values for this gene in all life-stages of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Mean FPKM value of all japonica control samples
This is the mean value of the N=13 RNASeq FPKM expression values for this gene in all life-stages of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Mean FPKM value of brenneri WBls:0000038 (L4) control samples
This is the mean value of the N=2 RNASeq FPKM expression values for this gene in this life-stage (L4) of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Mean FPKM value of brugia WBls:0000078 control samples
This is the mean value of the N=7 RNASeq FPKM expression values for this gene in this life-stage of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.
- Mean FPKM value of brugia WBls:0000081 control samples
This is the mean value of the N=23 RNASeq FPKM expression values for this gene in this life-stage of this species of all samples in the SRA that are wildtype controls that do not appear to have undergone any experimental conditions or treatment that would affect gene expression.