gene: algn-11 [Browse genome (BioProject PRJNA13758)] [Search on AGR]
Caenorhabditis elegans Predicted to enable GDP-Man:Man3GlcNAc2-PP-Dol alpha-1,2-mannosyltransferase activity. Predicted to be involved in dolichol-linked oligosaccharide biosynthetic process. Predicted to be located in endoplasmic reticulum membrane. Human ortholog(s) of this gene implicated in congenital disorder of glycosylation Ip. Is an ortholog of human ALG11 (ALG11 alpha-1,2-mannosyltransferase).
gene: sem-5 [Browse genome (BioProject PRJNA13758)] [Search on AGR]
Caenorhabditis elegans Enables epidermal growth factor receptor binding activity. Involved in several processes, including epidermal growth factor receptor signaling pathway; male genitalia development; and regulation of vulval development. Predicted to be located in cytoplasm; nucleoplasm; and plasma membrane. Predicted to be part of COP9 signalosome. Expressed in several structures, including P3.p hermaphrodite; P4.p hermaphrodite; P5.p hermaphrodite; P7.p hermaphrodite; and P8.p hermaphrodite. Human ortholog(s) of this gene implicated in autosomal recessive nonsyndromic deafness 114; breast cancer; and reproductive organ cancer (multiple). Is an ortholog of human GRB2 (growth factor receptor bound protein 2).
expression cluster: WBPaper00037901:GLD-1_mRNA_targets Authors calculated the mean IP enrichment from the two analyses and given a cutoff of three-fold, they identified 948 reproducibly enriched mRNAs (14.2%) from of a total of 6635 detected by the array.
948 reproductively enriched mRNAs that co-immunoprecipitate with GLD-1. To identify GLD-1 mRNA targets, authors performed immunoprecipitation (IP) of GLD-1, followed by microarray analysis of the co-IPed mRNAs (RIP-chip). Extracts from young adult transgenic worms expressing a rescuing FLAG and GFP-tagged GLD-1, hereafter referred to as tagged GLD-1, were subjected to IP in triplicate with anti-FLAG (aFLAG IP) or anti-MYC (aMYC IP) antibodies as controls. Comparison of aFLAG IP versus aMYC IP to input revealed a large population of GLD-1-associated transcripts. Authors additionally performed complementary aFLAG IPs upon worms expressing either tagged GLD-1(GGF IP) or non-tagged GLD-1(N2 IP). Comparing transcript 'IP-enrichment values' from both approaches revealed a correlation of 0.96, which indicated high reproducibility of GLD-1 association with specific mRNAs even on a quantitative level.