WBPaper00059852:B.malayi_iL3_stage_downregulated_vs_L3Day9

Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained. Transcripts that showed significantly decreased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L3 Day 9 worms.

WBPaper00059852:B.malayi_iL3_stage_downregulated_vs_L3Day6

Differential gene expression analysis was performed using both DESeq and EdgeR, and the overlapping genes with FDR<0.01 were retained. Transcripts that showed significantly decreased expression at infective lava stage 3 (iL3) (from mosquitoes), comparing to L3 Day 6 worms.

WBPaper00048706:digestive-tract_enriched

Protein quantitation was determined by normalized spectral abundance.Proteins with NSAF enrichment values of 2 or greater were were considered enriched within that fraction. Proteins that are enriched in digestive tract of adult female Brugia malayi.

WBPaper00049974:ivermectin_300nM_5-day_upregulated

Differential gene expression analysis between time points and drug concentrations was realized in edgeR (version 3.10.5) through the web interface provided by NetworkAnalyst. Significance was set as an experiment-wide false discovery rate (FDR) <0.20 (after the Benjamini-Hochberg method). Transcripts that showed significantly increased expression after 5 days of 300nM ivermectin exposure.

WBPaper00049974:ivermectin_100nM_48h_downregulated

Differential gene expression analysis between time points and drug concentrations was realized in edgeR (version 3.10.5) through the web interface provided by NetworkAnalyst. Significance was set as an experiment-wide false discovery rate (FDR) <0.20 (after the Benjamini-Hochberg method). Transcripts that showed significantly decreased expression after 48 hours of 100nM ivermectin exposure.

WBPaper00051007:upregulated_F30_vs_L4

EdgeR(v3.16.5), FDR < 0.05. Transcripts that showed significantly increased expression in female B. malayi 30 days after infecting gerbils, comparing to L4 female B. malayi animals.

WBPaper00048706:productive-tract_only

Protein quantitation was determined by normalized spectral abundance.Proteins with NSAF enrichment values of 2 or greater were were considered enriched within that fraction. Proteins that are only expressed in productive tract of adult female Brugia malayi.

WBPaper00048706:productive-tract_enriched

Protein quantitation was determined by normalized spectral abundance.Proteins with NSAF enrichment values of 2 or greater were were considered enriched within that fraction. Proteins that are enriched in productive tract of adult female Brugia malayi.

WBPaper00041323:female-associated

Criteria used for protein identification included: two or more peptide matches, MASCOT score > 60, and P < 0.05. Proteins that were enriched in female animals.

WBPaper00045439:BM_Cluster_P3

Non-flat profiles (p < 0.01 and maximum fold-difference among time points > 2) were grouped into common temporal patterns using k-means clustering. Time-course transcript abundance profiles. P116, k-means clusters of Brugia malayi during development in Aedes aegypti LVP between day 1 and 8 post infection; H114, k-means clusters of Ae. aegypti LVP infected with B. malayi between day 0 and 8 post infection; and HR14, host response profile comparing infected vs. uninfected A. aegypti LVP.