GLW39

Caenorhabditis elegans

MIR276

Caenorhabditis elegans

WBPaper00040722:GPI-anchored_protein

Statistical analyses, including one-way analysis of variance, followed by Holms multiple-comparison test, were performed using R statistical package (R, version 2.11.0) Glycosylphosphatidylinositol (GPI) anchored proteins identified using fluorochrome (Alexa 488) labeled inactivated aerolysin (FLAER) staining, fractionation, SDS-PAGE, liquid chromatography and mass spectrometry.

WBPaper00035654:alpha-synuclein_regulated

TIGR Spotfinder software was used to filter unreliable spots based on Otsu's method algorithm and to convert good spots in microarray images to numerical form. Raw signal values were analyzed using mean centering to normalize signal values between channels. Signals from Alexa Fluor Dye Control probes were excluded from normalization process due their oversaturation leaving only probe intensities from C. elegans miRNAs to use in normalization. Signal intensity ratios were calculated for each signal pair yielding fold change values and p values for each miRNAs from the strain of investigation. A cutoff of 1.4 was used to detect modestly changing miRNAs. Genes that differentially expressed in WBPaper00035654Is1[aex-3

WBPaper00035654:cat-1(e1111)_regulated

TIGR Spotfinder software was used to filter unreliable spots based on Otsu's method algorithm and to convert good spots in microarray images to numerical form. Raw signal values were analyzed using mean centering to normalize signal values between channels. Signals from Alexa Fluor Dye Control probes were excluded from normalization process due their oversaturation leaving only probe intensities from C. elegans miRNAs to use in normalization. Signal intensity ratios were calculated for each signal pair yielding fold change values and p values for each miRNAs from the strain of investigation. A cutoff of 1.4 was used to detect modestly changing miRNAs. Genes that differentially expressed in cat-1(e1111) comparing with N2 at L4 larva.

WBPaper00035654:pdr-1(gk448)_regulated

TIGR Spotfinder software was used to filter unreliable spots based on Otsu's method algorithm and to convert good spots in microarray images to numerical form. Raw signal values were analyzed using mean centering to normalize signal values between channels. Signals from Alexa Fluor Dye Control probes were excluded from normalization process due their oversaturation leaving only probe intensities from C. elegans miRNAs to use in normalization. Signal intensity ratios were calculated for each signal pair yielding fold change values and p values for each miRNAs from the strain of investigation. A cutoff of 1.4 was used to detect modestly changing miRNAs. Genes that differentially expressed in pdr-1(gk448) comparing with N2 at L4 larva.