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Comments on Bah-Tahe, M. et al. (2019) International Worm Meeting "Roles of heparan sulfate proteoglycans in the development of polarized cells." (0)
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Bah-Tahe, M., Dima, R., Rivollet, L., Shaye, D., & Benard, C. (2019). Roles of heparan sulfate proteoglycans in the development of polarized cells presented in International Worm Meeting. Unpublished information; cite only with author permission.
The regulation of cell shape and polarity is essential to animal development and physiology. Heparan sulfate proteoglycans (HSPGs) shape the interactions of morphogens and guidance cues with their respective receptors to elicit appropriate cellular responses. HSPGs consist of a protein core with attached heparan sulfate glycosaminoglycan chains, which are synthesized by glycosyltransferases of the exostosin (EXT) family. Abnormal HS chain synthesis results in pleiotropic consequences, including abnormal development and tumor formation. Complete loss of any of the exostosin glycosyltransferases in mouse, fish, flies and worms leads to drastic morphogenetic defects and embryonic lethality. Using our viable hypomorphic mutations in the two C. elegans exostosin glycosyltransferases genes, rib-1 and rib-21, we are dissecting the roles of HSPGs in regulating the establishment of proper morphology of polarized cells. In particular, rib-1 and rib-2 mutants display supernumerary cellular projections in neurons and the excretory cell. Using molecular genetics, we are addressing the cellular and molecular mechanisms that regulate the number of polarized cell projections and the roles of HSPGs and their interactors in this process. 1. Blanchette, Thackeray, Perrat, Hekimi, Benard (2017) PLOS Genetics
Affiliations:
- Biology, Universite du Quebec a Montreal, Montreal, Qc, CA
- Dept Physiology and Biophysics, University of Illinois at Chicago, USA