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Resources » Paper

Y Sasaki et al. (1999) International C. elegans Meeting "Differential expression of CeCRMP/DHP-1 and CeCRMP/DHP-2, novel members of CRMP/DRP/DHP/UNC-33 family in C. elegans."

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  • Comments on Y Sasaki et al. (1999) International C. elegans Meeting "Differential expression of CeCRMP/DHP-1 and CeCRMP/DHP-2, novel members of CRMP/DRP/DHP/UNC-33 family in C. elegans." (0)

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00023608

    Y Sasaki, T Takemoto, H Kimura, M Nonaka, & Y Goshima (1999). Differential expression of CeCRMP/DHP-1 and CeCRMP/DHP-2, novel members of CRMP/DRP/DHP/UNC-33 family in C. elegans presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    Collapsin is recognized as a member of a semaphorin/collapsin gene family which is thought to be involved in axonal guidance. We isolated a collapsin response mediator protein (CRMP) from chick dorsal root ganglion, using Xenopus laevis oocyte expression system. In rodents, 4 subtypes of CRMP are expressed mainly in the nervous system during development. Independently, we cloned 4 human dihydropyrimidinase (DHP)-related proteins (DRP-1-4), human homologues of CRMP, by their homology to DHP. DHP is the second enzyme in the pyrimidine degradation pathway. However, all CRMP/DRPs have replacement at one or more essential residues for DHP activity, and CRMP/DRP purified from brain does not show any DHP activity. Therefore, a role of CRMP/DRPs in axonal guidance remains to be elucidated. Interestingly, chick CRMP/DRP shares 33% amino acid identity with the C. elegans UNC-33. unc-33 nematodes move in an uncoordinated fashion, and axon outgrowth is aberrant. In database of C. elegans Genome Project, we here identified two additional genes of CRMP/DRP/DHP/UNC-33 homologues. These two gene products shared similar amino acid identities with chick CRMP/DRP (40-46%) and rat DHP (49-55%), and thus these genes were termed CeCRMP/DHP-1 and -2 . To examine the expression of these related genes, we constructed CeCRMP/DHP::GFP (green fluorescent protein) transgenes, which were driven under control of CeCRMP/DHP native upstream genomic sequences. The CeCRMP/DHP-1::GFP was expressed in some neuronal cells and hypodermis in larval stage, but was downregulated in adult stage. On the other hand, the expression of CeCRMP/DHP-2::GFP was seen predominantly in body wall muscle cells at 180-cell stage and continued throughout life.


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