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Resources » Paper

Kohler R et al. (1997) International C. elegans Meeting "MAPPING THE CEH-13 ENHANCER"

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    Status:
    Publication type:
    Meeting_abstract
    WormBase ID:
    WBPaper00022455

    Kohler R, Fleischmann M, Brunschwig K, Tobler H, & Mueller F (1997). MAPPING THE CEH-13 ENHANCER presented in International C. elegans Meeting. Unpublished information; cite only with author permission.

    Metazoan development is in part regulated by a group of homeobox containing genes arrayed in an evolutionarily conserved cluster. ceh-13, a member of the C. elegans HOM-C cluster, is the orthologue of the Drosophila labial gene. Antibody staining and reporter gene expression experiments have revealed that CEH-13 has a highly complex and dynamic expression pattern (see also posters by Fleischmann M. and Brunschwig K.). Additionally, these studies identified an 8 kb upstream enhancer/promoter region that was sufficient to confer essentially wild type expression to GFP reporter gene constructs. Different biochemical and genetic aspects of the regulation of Hox/HOM-C expression have been described in various species. Nevertheless a complete picture of Hox/HOM-C transcriptional control awaits to be put forward. Furthermore, recent studies indicated that the control of these genes in C. elegans may be significantly different from what is known from other species. For those reasons, we initiated a detailed in vivo analysis of the ceh-13 enhancer region by means of expression monitoring of various GFP reporter gene constructs in transgenic animals. So far these studies have shown that different, dissectable enhancer elements are present in the ceh-13 regulatory region. These elements appear to independently direct different aspects of CEH-13 expression. A preliminary analysis of different enhancer elements and their significance in the context of C. elegans development will be presented.

    Affiliation:
    - Institute of Zoology, University of Fribourg, Perolles, CH-1700 Fribourg


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